PRRT2 and glaucoma: Propagates Ca2+ bursts during retinal development [198,199].G protein-coupled mAChRs activate the alpha subunits of Gq/11 proteins, raising the levels of intracellular Ca2+. This Ca2+ increase enhances PKC activity and may stimulate NO production via Ca2+-dependent neuronal nitric oxide (NO) synthase [200].M2 and M4 receptors inhibit the cAMP-dependent pathway, indirectly influencing Ca2+ dynamics [200].Associated with neuroprotective roles, preserving Ca2+ balance and mitochondrial membrane integrity with drugs such as pilocarpine, which is effective against glaucoma [35].