In addition, as illustrated in Figure 7F (IBS gut/degenerative brain axis), the phosphorylation of SIRT1 induced by VitD3 NS (p < 0.05) was increased compared to LPS 100 ng/mL + H2O2 200 μM (43.5%, p < 0.05) and the other forms of VitD3 (22% vs. VitD3 Physical Mix 100 nM and 16.5% vs. VitD3 Sigma 100 nM, p < 0.05), supporting the efficacy of VitD3 NS in increasing the presence of this molecule. This evidence concerns the gene SIRT1 and irritable bowel syndrome.