The positive effect of VitD3 NS was also supported by APP level (Figure 7D, IBS gut/degenerative brain axis); indeed, treatment with VitD3 NS 100 nM reduced the APP level about 40% vs. LPS 100 ng/mL + H2O2 200 μM (p < 0.05), about 19.5% vs. VitD3 Physical Mix 100 nM (p < 0.05), and about 16% vs. VitD3 Sigma 100 nM (p < 0.05). Here, APP is linked to irritable bowel syndrome.