The median LOD for SERS-based IA methods for detecting this cancer biomarker was 6.0 × 10−16 M, while for fluorescent spectroscopic IA detection, the median LOD was 3.2 × 10−12 M. If in the detection of AFB1, AFP and cTnI, methods using SERS was more sensitive and the median LOD was smaller, then in the case of OTA, fluorescent spectroscopic detection was more sensitive. The gene discussed is AFP; the disease is cancer.