More hypo-methylated regions were observed on chromosomes 2, 3, 5, 11, 15, and X, and these downregulated methylation genes were enriched in pathways associated with T2DM and cell signalling, such as transcriptional misregulation in cancer, hypertrophic cardiomyopathy, Hippo signalling pathway, mTOR signalling pathway, basal cell carcinoma, longevity regulating pathways-multiple species, FoxO signalling pathway, Toll-like receptor signalling pathway, signalling pathways regulating pluripotency of stem cells, and TNF. The gene discussed is TNF; the disease is type 2 diabetes mellitus.