Given the potential of PET, MRI, and radiomics in glioma assessment, this study aimed to compare and evaluate the predictive value of radiomics derived from TSPO PET, static and dynamic [18F]FET PET, and T1- and T2-weighted MRI for determining the IDH mutation status in glioma patients at the time of initial diagnosis. Here, IDH1 is linked to central nervous system cancer.