BUB1 and in situ carcinoma: In our analysis, we found TGCT to be the only tumour exhibiting BUB1 downregulation; while BUB1 was not assessed specifically, expression of spindle genes was shown to correlate with TGCT histological subtypes, as observed by differential expression patterns (up- or downregulation) in in situ carcinomas, seminomas or non-seminomas86.