First, we performed Kaplan–Meier analyses using expression levels of BUB1 (“high” or “low” based on mean cut-offs) by cancer site and found that a high expression of BUB1 resulted in worse overall survival (OS) in LUAD, UCEC, PAAD, SKCM, LIHC, ACC, KIRC, KIRP, and LGG (Supplementary Fig. 2). Here, BUB1 is linked to cancer.