Hence, we examined the function of 6 unfavorable prognostic risk factors in the viability of glioma cells (U87 and U251 cells), which revealed that silencing BGN, SDC1, SERPINA1, TUBA1C, C1GALT1C1L and SPTBN5 obviously inhibited the cell viability of U87 cells (Fig. 9A–F, p < 0.05, p < 0.01, p < 0.001) and U251 cells (Supplementary Fig. 6, p < 0.01, p < 0.001). Here, SERPINA1 is linked to central nervous system cancer.