In order to further investigate the biological role of SLC12A2 in CRC cells, two specific SLC12A2 siRNAs (siSLC12A2 #1, #2) were used to knockdown SLC12A2 expression in HT29 and LOVO cells while pCMV3-SLC12A2 plasmids were used to overexpress SLC12A2 expression in HCT116 and SW480 cells. Here, SLC12A2 is linked to colorectal carcinoma.