The knockdown efficiency of shHIGD1A was confirmed in Fig. 2A. First, we examined the cell viability using CCK-8 assay, which revealed that the knockdown of HIGD1A strongly inhibited the proliferation capacity of HCC cell lines while having no discernible impact on L02 cells (Fig. 2B). The gene discussed is HIGD1A; the disease is hepatocellular carcinoma.