In assessing tumor angiogenesis, the D* value showed the highest correlation with CD31 (r = 0.702, P = 0.001), the D value exhibited the highest correlation with α-SMA (r = 0.749, P < 0.001), and the f value demonstrated the highest correlation with vessel maturity (r = 0.693, P = 0.001). This evidence concerns the gene PECAM1 and neoplasm.