TGFB1 and Hepatic fibrosis: To investigate the mechanism by which miR-541 inhibited the activation of HSCs, we detected the effects of miR-541 on the luciferase activities of reporters of liver fibrosis-associated signaling pathways, including Notch, TGF-β, Hedgehog, Hippo, Wnt, and NF-κB signaling in LX2 cells.