To specifically address our primary aim of examining whether the SecA2 protein secretion system contributes to an RNA driven, IFN-β mediated pathogenesis mechanism in M. marinum as it does in M. tuberculosis, we infected WT and MAVS-/- BMDMs with our generated strains and examined the macrophage secretome for secreted levels of IFN-β 24-hours post infection. The gene discussed is IFNB1; the disease is infection.