For example, Vimentin+CD133- HCC cells demonstrate EMT phenotypes whereas Vimentin-CD133+ stem cells do not have EMT phenotypes, and Vimentin-CD133- cells exhibit more aggressive metastasis than vimentin-CD133+ cells.33 Therefore, the correlation between HCC stem cells and the EMT requires further investigation. This evidence concerns the gene PROM1 and hepatocellular carcinoma.