Since we examined infection parameters in WT and Nlrp3−/− mice up to 72 h after the induction of ascending UTI without a catheter, future experiments focused on determining (i) whether MRSA-induced NLRP3 activity induces exfoliation of the uroepithelium, in turn promoting chronic MRSA-UTI, similar to what is reported in UPEC-UTI, and (ii) whether urinary NLRP3 activity shapes the pathophysiology of life-threatening exacerbations such as uBSI following MRSA CAUTI are warranted. This evidence concerns the gene NLRP3 and infection.