NLRP3 and cystitis: Indeed, 24 h after experimental induction of ascending UTI with UPEC CFT073, Nlrp3−/− mice exhibited significantly higher UT bacterial burden and severe cystitis compared with their wild-type (WT) counterparts, although in UPEC-infected Nlrp3−/− mice, while IL-1β was processed via a non-canonical, metalloproteinase-7-mediated mechanism [23].