BTG3 and systemic lupus erythematosus: The OS and IS of the duplex ANA–αDNA VFA showed good discriminating power for distinguishing SLE patients from HCs, as did the commercial ELISA, based on ROC AUC analysis, as shown in Figure 4A. The αDNA levels in SLE patients were significantly higher than those in HCs when assayed via ELISA (p < 0.05, Figure 4B,C), or via the VFA (p < 0.0001, Figure 4D).