TNF and neoplasm: Of note, GSDME-deficient tumors exhibited reduced levels of tumor-associated macrophage (TAMs) and tumor infiltrating lymphocytes (TILs) such as CD8+ T and NK cells, which expressed less perforin and GzmB, and had attenuated levels of IFNγ (interferon gamma) and TNFα compared to the GSDME-expressing tumors [60].