HLA-F and glioblastoma: Avoiding HLA-F monoclonal antibodies, Hrbac et al. [51] detected HLA-F overexpression in Glioblastoma (GBM) by comparing the expression of 69 GBM and 21 non-tumor brain tissue samples using RT-qPCR or a high-capacity cDNA reverse transcription polymerase (ThermoFisher Scientific) assay.