The effect of total ligand expression on the frequency of TLS-infiltrating CD8+ T cells was investigated, total tumor-infiltrating CD8+ T cells and the exhausted CD8+T cells (TIM-3+/LAG-3+/PD-1+/PD-1+TIM-3+CD8+T cells) were analyzed, results showed that a higher total checkpoint ligand expression in HEVs represented a lower percentage of TLS-infiltrating CD8+ T cells, whereas a lower total ligand expression indicated increased CD8+ T cell infiltration (Figure 5B), with the differences being statistically significant. Here, LAG3 is linked to neoplasm.