In addition, the prevalence of systemic autoantibodies was assessed for RF, ANA, and, in case of positive ANA, anti-ENA autoantibodies, a subset of ANA used to diagnose, differentiate, and monitor various autoimmune disorders and associated with different CTDs, including SLE, Sjögren’s syndrome, and different forms of systemic sclerosis (23). This evidence concerns the gene ENAH and autoimmune disease.