To elucidate the impact of TCF7L2 on the ccRCC biological functions, stable cell lines with TCF7L2 knockdown and overexpression were established in A498 and 786-O cells using lentiviral infection with TCF7L2 shRNA or overexpression vectors (Fig. 2A and B). Here, TCF7L2 is linked to nonpapillary renal cell carcinoma.