Consistent with the finding, in the present study, OCT4-binding inhibition in the intron 1 region of MYCN decreased the proliferation of MYCN-amplified NB cells (CHP134 and IMR32) but not that of MYCN-non-amplified NB cells (SK-N-AS), further suggesting that the human-specific OCT4–MYCN network is specifically required for the survival of MYCN-amplified NB. This evidence concerns the gene POU5F1 and neuroblastoma.