Finally, we tested the abundance of p22phox (CYBA), gp91-phox (CYBB), NCF1, NCF2, and NCF4 - membrane and cytoplasmic components of the NADPH-oxidase complex, and we did not observe any difference between HVs, CKD and CVD groups (Supplementary Figure S3). This evidence concerns the gene NCF4 and chronic kidney disease.