Due to the high abundance of 20-HETE in lesional skin of our CD model mice, as revealed by UHPLC, and its potency in activating TG neurons (Figure S10A-F), we next examined if skin derived 20-HETE is a major contributor of TRPV1-mediated itch and pain in CD disorder. The gene discussed is TRPV1; the disease is Cowden disease.