The present study revealed that MOTS-c downregulation correlated with JNK pathway activation in ECs, in accordance with previous studies showing that MOTS-c inhibited JNK function in formalin-induced inflammatory nociception 19, lipopolysaccharide-induced acute lung injury 57 and lipopolysaccharide-induced septic cardiomyopathy 58. This evidence concerns the gene MT-RNR1 and acute lung injury.