In the bioinformatic analysis, each configuration of the new HD-tDCS application revealed regulation of specific genes related to stroke insults, including Insig1, Bag5, Nfkb1, Bche, Egr2, Hdac2, Sirt1, and U2af2. Since most of these genes are involved in brain injury through cell death after stroke, we examined the apoptotic markers. Here, NFKB1 is linked to Stroke.