These results suggest that both electrode configurations of HD-tDCS manifested specific gene regulation related to stroke insults, and we selected potent genes related to brain injury and protection after stroke, such as Insig1, Bag5, Nfkb1, Bche, Egr2, Hdac2, Sirt1, and U2af2. The gene discussed is NFKB1; the disease is Stroke.