As a proof of principle, we induced genetic deficiencies for DMD (Duchenne muscular dystrophy, OMIM#310200) and CAPN3 (limb-girdle muscular dystrophy type 2A (LGMD2A), OMIM#253600) in healthy hiPSC-based 3D-TESMs. Here, CAPN3 is linked to Duchenne muscular dystrophy.