PLIN5 and metabolic dysfunction-associated steatotic liver disease: Furthermore, TAG analysis did not reveal significant differences between the Plin5−/− and WT groups in both models when fed WD, while cholesterol levels of Plin5−/− mice fed WD were reduced in the NAFLD model (p < 0.01, two-way ANOVA using Tukey’s post-test) (Fig. 2C).