CEP250 and colorectal carcinoma: Additionally, we over-expressed Nek2 in U2OS cells that were either wild type, C-Nap1 KO, or Rootletin KO (Fig. 5A) in order to assess: (i) how increased levels of Nek2 affect the separation of CRCs, and (ii) whether centrosomal linkers are necessary for Nek2-mediated CRC separation.