To determine whether the Met+Rap treatment of CAR-T cells improves their functions in vivo, C57BL/6J mice bearing intracerebral SB28 mEGFRvIII gliomas received a single i.v. infusion of 1 × 106 CAR-T cells pretreated with Met or Rap alone, Met+Rap, or without any of these (Figure 4A). This evidence concerns the gene LRPAP1 and glioma.