Genes regulating stem cell maintenance, such as POU5F1 and NANOG, were in Cluster I, and genes such as HES5 and NEUROG2, which play important roles in neuronal differentiation, were observed in Cluster N. To determine the relationship between chromatin accessibility and the expression of associated genes, we generated RNA-seq data for iPSC and NPC states. Here, NANOG is linked to nasopharyngeal carcinoma.