We also evaluated the protein expression of epithelial-mesenchymal transformation (EMT) (N-cadherin, E-cadherin, Vimentin, Twist1) and cell stemness-related biomarkers (Bmi1, Nanog, CD44) in GC cells that were co-cultured with EGR1-overexpressing and EGR1-knockout HMrSV5 cells. The gene discussed is NANOG; the disease is gastric cancer.