Furthermore, the interference of PMEPA1 increased bladder cancer cells' apoptosis rate, the level of apoptosis-related proteins Cleaved-caspase3, Cleaved-PARP expression, decreased the caspase3, PARP expression, which implied it can reverse the anti-apoptosis induced by METTL16 knockdown in T24 and UMUC3 (Figure 6H,6I). This evidence concerns the gene PMEPA1 and urinary bladder cancer.