At the same time, all results confirmed that the expression of METTL16 and PMEPA1 was negatively correlated in bladder cancer tissues and cell lines, which may affect the proliferation and drug resistance of bladder tumor cells through PMEPA1 mediated autophagy, which also provided clues for the sensitization of traditional comprehensive therapy by inhibiting PMEPA1 and autophagy in bladder cancer. Here, METTL16 is linked to urinary bladder cancer.