Similarly, 24 hours of 500 μM SAMe treatment (500 μM SAMe was used for cancer cells since 1 mM may induce cell death18) significantly reduced MCJ protein levels in HepG2, Huh-7 and SAMe-D cells (Fig. 3A-B) without altering MCJ at the mRNA level (Fig. 3C). This evidence concerns the gene DNAJC15 and cancer.