ISG15 and infection: To shed light whether ISG15 might be affecting viral entrance or early during the viral cycle, DV infection at 4 °C for 2 hours followed by relative intracellular DV genome quantification indicated no differences in viral RNA between WT and knockout cells up to 48 hours post-infection (Figure 2G), indicating that ISG15 plays a role in the DV life cycle at a stage after viral entry.