The results showed that compared with the control group, the expression levels of PI3K and AKT protein in CRC cells treated with AIL did not show any significant change (Supplementary Figures S2, S3), but the expression level of p-AKTS473 protein was significantly decreased (p < 0.01), indicating that AIL could inhibit AKT phosphorylation, thereby inhibiting the activation of the PI3K/AKT signaling pathway and thus inhibiting the proliferation and metastasis of CRC cells, as shown in Figure 8. Here, AKT1 is linked to colorectal carcinoma.