To validate the role of HMGA1 in the regulation of ferroptosis in ESCC cells, we induced ferroptosis in HMGA1-manipulated ESCC cells with erastin, a small molecule capable of initiating ferroptotic cell death by inhibiting the cystine-glutamate antiporter system Xct− and glutathione synthesis. Here, SLC7A11 is linked to esophageal squamous cell carcinoma.