To validate the role of HMGA1 in the regulation of ferroptosis in ESCC cells, we induced ferroptosis in HMGA1-manipulated ESCC cells with erastin, a small molecule capable of initiating ferroptotic cell death by inhibiting the cystine-glutamate antiporter system Xct− and glutathione synthesis. This evidence concerns the gene HMGA1 and esophageal squamous cell carcinoma.