T cells transduced with the HuJovi-1 and KFN CARs were co-cultured with cell lines that either endogenously expressed TRBC1 (Jurkat and H9) or TRBC2 (HPB-ALL, T-ALL1 and HD-MAR2), or were engineered to express TRBC1, TRBC2 or TCR KO (Jurkat and HPB-ALL). The gene discussed is TRBC2; the disease is acute lymphoblastic leukemia.