ABCB11 and metabolic dysfunction-associated steatotic liver disease: Although the observed periportal increases were slightly greater in magnitude than opposing pericentral decreases (0.058 vs. −0.042), this would theoretically result in a largely negligible net change in overall BSEP liver abundance, consistent with a previous report of no change in overall BSEP abundance in NAFLD measured by quantitative targeted absolute proteomics.18 These results support the validity of our approach in obtaining zonal data and demonstrate the value of zonal assessment to better understand NAFLD pathophysiology.