The primary aim of this study was to quantitatively assess the zonal abundance (tissue level) and plasma membrane localization (cellular level) of 4 key hepatocyte BA transporters (BSEP, NTCP, OATP1B1, and OATP1B3) in non-diseased livers (NDLs) and livers at various stages of NAFLD progression. Here, SLC10A1 is linked to metabolic dysfunction-associated steatotic liver disease.