We first focused on a subset of genes that we have shown previously to be required for the activation of an atheroprotective programme in MZB cells in response to HF/HC diet.9,20 MZB cells from No Tfh mice had a significant decrease of transcription factors Atf3 and Nr4a1, and the surface protein PDL1, as well as their upstream regulators, including the B cell receptor and Toll-like receptor (Btk, Tlr6) signalling pathways (Figure 3B–D). This evidence concerns the gene BTK and hydrops fetalis.