TARDBP and bone osteosarcoma: To further confirm that the inhibition of RNA-binding has a direct influence on hTDP-43 LLPS propensities, we transfected human bone osteosarcoma epithelial cells (U2OS; selected due to their favorable microscopy qualities) with human TDP-43 5FL, an RNA-binding deficient mutant with an additional phenylalanine to leucine mutation at amino acid 194 (9,25).