In previous studies, we found that the deposition of calcium‐containing crystals in mouse kidneys could be inhibited by suppressing the level of ferroptosis in the kidneys of the kidney stone model.[22, 25] In this study, we successfully demonstrated the effectiveness of the single‐vector CRISPR‐dRfxCas13d‐eIF4G translational regulatory tool in mammalian models in vivo, and have applied this gene tool for the first time to the best of our knowledge to treat kidney stones. The gene discussed is EIF4G1; the disease is nephrolithiasis.