Five types of organic acid metabolic diseases (short/branched chain acyl-CoA dehydrogenase deficiency (SCADD), methylmalonic cademia (MMA), middle/branched chain acyl-CoA dehydrogenase deficiency (MCADD), holocarboxylase synthetase (HCSD), and glutaric cademia type I (GA-I) were detected. This evidence concerns the gene HLCS and 2-methylbutyryl-CoA dehydrogenase deficiency.