KRAS and colorectal carcinoma: The fully automated IdyllaTM RAS mutation method is a qPCR test that is performed in two separate consecutive runs with a reported analytical sensitivity rate of ≤1% for tumors harboring hotspot KRAS point mutations in exons 2 and 3 and ≤5% for CRC-associated rare mutations centered on KRAS exon 4 [84, 93].