The NGS analysis was carried out in Geneseeq Technology Inc. using a 6-gene targeted panel (EGFR, ALK, ROS-1, BRAF, KRAS, and HER2), which identified the simultaneous presence of two fusion genes PLEKHA7-ALK (P18:A20, allelic frequency: 16.60%) and INPP5D-ALK (I2:A19, allelic frequency: 13.92%) in the pleural effusion sample (Fig. 2). This evidence concerns the gene ALK and Pleural effusion.