We have also determined antiproliferation effects in human cancer cell lines H1975 and HCC827 treated with 4, which show that this EGFR inhibitor is active at concentrations ~100–500 nM overall ~60-fold less potent than AZD9291 in both cell lines (Supplementary Table S5, Supplementary Fig. S18). The gene discussed is EGFR; the disease is cancer.