Inhibition of IKKβ activity in P. obesus protects against diet-induced diabetes and decreases IL-1β induced ROS detected by employing the 2′,7′-Dichlorodihydrofluorescein diacetate (DCFH-DA) method (mainly HO•, H2O2, and the peroxynitrite anion [ONOO−]/radical [ROO•] [146]), loss of insulin production, and beta-cell death in vitro [147]. Here, INS is linked to diabetes mellitus.