NOS3 and Hyperglycemia: Inconsistent with the increased glutathionylation of eNOS in hyperglycemic rabbit aorta, co-immunoprecipitation of eNOS/Grx1 and β1subunit/Grx1 was significantly diminished under hyperglycemic conditions [202], suggesting that hyperglycemia may impair Grx1 activity and increase eNOS glutathionylation.