To identified which receptor mediating the effect of CIRP on NETs formation in ischemic stroke, we detected the expression of TLR4 and TREM1 in peripheral neutrophils isolated from tMCAO mice (24 hours) and found that the TLR4 expression was significantly elevated, whereas TREM1 was only increased slightly (Supplementary Fig. 6A-B). Here, TLR4 is linked to ischemic stroke.