The top two gene DEFB103B (defensin beta 103) functioned as antimicrobial peptide that counteracts Staphylococcus and reduces Mycobacterial infection capacity (37, 38), which was confusing because the purpose of negative selection was infection control, but gene function suggested the presence of DEFB103B, not knockout, should have induced bacterial death. This evidence concerns the gene DEFB103B and infection.