On the one hand, researchers can identify tumor-specific genetic alterations, gene effects on immune infiltration, gene effects on survival and gene effects on drug sensitivity by visualization and analysis of genes, samples, and clinical data33–36; on the other hand, researchers can identify gene effects on cell migration, invasion and proliferation by in vitro and in vivo experiments.37,38 In this study, TCGA expression and clinical database analysis and in vitro/vivo experiments were performed to further verify that MDFI was an effective CRC diagnostic and therapeutic target. Here, MDFI is linked to colorectal carcinoma.