To investigate the effect of MDFI on the progression of CRC in vitro and in vivo, we transfected MDFI-knockdown shRNA (shMDFI#1, shMDFI#2) and MDFI-overexpressing lentiviral vector plasmids (LvMDFI) into HCT116 and SW620 cells to construct MDFI-dysregulated CRC cells. Here, MDFI is linked to colorectal carcinoma.