In recent years, some new technologies have been used to search for these targets, among which single-cell sequencing and PPIs have revolutionized cancer research.26,27 Using single-cell sequencing, Yuan Zhou et al. identified prevalent genomic alterations in the tumor stroma of CRC patients.28 Kennedy SA et al. discovered effective CRC molecular targets and their network in CRC cells by PPIs.29 In this article, we performed detailed analyses of single-cell sequencing data from the GSE144735 by PPIs and revealed that MDFI was a potential CRC therapeutic target. Here, MDFI is linked to colorectal carcinoma.