- Robo1 and Robo4 are significantly upregulated in colorectal carcinoma.- Slit2 and Robo1 are associated with an increased metastatic risk and decreased survival in colorectal carcinoma patients.- Increased Slit1 expression in prostate tumors- Slit/Robo signaling pathway plays a role in enhancing tumor cell migration and promotes tumor metastasis.- Slit2/Robo1 signaling upregulates MMP-9 to enhance breast cancer cell invasion.- Slit/Robo signaling decreases the proliferative rate and increases the apoptotic rate of oral squamous cells. Here, ROBO4 is linked to prostate neoplasm.